Uptake, accumulation and metabolism of UV-320 in vegetables and its impact on growth and quality.

UV-320 is classified as a Substance of Very High Concern (SVHC) by the European Chemicals Agency and has attracted significant attention due to its presence in the environment. Understanding the uptake, translocation and metabolic patterns of UV-320 in vegetables is essential for assessing their ability to bioaccumulate and potential risks to human health. In this study, we investigated the uptake and translocation of UV-320 in lettuce and radish by hydroponic experiments. The results showed that the root concentration factors (Croot/Csolution, RCF) of lettuce and radish were in the range of 47.9 to 464 mL/g and 194 to 787 mL/g, respectively. The transfer factors (Cshoot/Croot, TF) were observed to be 0.001-0.012 for lettuce and 0.02-0.05 for radish. Additionally, non-targeted screening identified twelve phase I and one phase II metabolites of UV-320 in vegetables, which were confirmed based on their molecular formulas and structures. The metabolic pathways involving oxidation, ketonylation and deamination were proposed in vegetables. Also, we have observed that UV-320 inhibits the growth of vegetables. Meanwhile, we evaluated the health risk of UV-320 in lettuce and radish and found that the consumption of lettuce is relatively safe, while the consumption of radish has a risk of HQ >1 for both adults and children, which should be seriously considered. This study provides valuable insights into the behavior and ecological risks of UV-320 in the environment.

Constructing graphene oxide/Au nanoparticle cellulose membranes for SERS detection of mixed pesticide residues in edible chrysanthemum.

Edible chrysanthemum is widely cultivated and used as an important ingredient of medicine, tea and multifunctional food. During the planting of chrysanthemum, pesticides are extensively used for preventing plant diseases and insect pests. To ensure the food safety of edible chrysanthemum, rapid detection methods are urgently needed for on-site inspection. In this study, a graphene oxide/Au nanoparticle (GO/Au NP) cellulose substrate was prepared through layer-by-layer assembly of GO and Au NPs on a mixed cellulose ester membrane. Surface-enhanced Raman spectroscopy (SERS) detection of four types of organophosphorus and organosulfur pesticides was achieved by filtering the extracting solution through the substrate and analysing SERS spectra. Qualitative and semi-quantitative detection of fenthion, phoxim, isocarbophos and thiram was accomplished with the detection limits of 38.01, 8.13, 48.97 and 8.74 ng mL-1, respectively. A spiking experiment further demonstrated the feasibility of this method for rapid and on-site detection of mixed pesticides in chrysanthemum. This study provides a new approach for rapid detection of multiple hazardous substances in flowering and herbal plants.

Biosensors based on core-shell nanoparticles for detecting mycotoxins in food: A review.

Mycotoxins are toxic metabolites produced by fungi in the process of infecting agricultural products, posing serious threat to the health of human and animals. Thus, sensitive and reliable analytical techniques for mycotoxin detection are needed. Biosensors equipped with antibodies or aptamers as recognition elements and core-shell nanoparticles (NPs) for the pre-treatment and detection of mycotoxins have been extensively studied. By comparison with monocomponent NPs, core-shell nanostructures exhibit unique optical, electric, magnetic, plasmonic, and catalytic properties due to the combination of functionalities and synergistic effects, resulting in significant improvement of sensing capacities in various platforms, such as surface-enhanced Raman spectroscopy, fluorescence, lateral flow immunoassay and electrochemical sensors. This review focused on the development of core-shell NPs based biosensors for the sensitive and accurate detection of mycotoxins in food samples. Recent developments were categorised and summarised, along with detailed discussion of advantages and shortcomings. The future potential of utilising core-shell NPs in food safety testing was also highlighted.

Advances in gold nanoparticles for mycotoxin analysis.

Mycotoxins are toxic secondary metabolites naturally produced by fungi. They can cause various kinds of acute and chronic diseases in both humans and animals since food usually contains trace amounts of mycotoxins. Thus, it is important to develop a rapid and sensitive technique for mycotoxin detection. Except for the original and classical enzyme-linked immunosorbent assays (ELISA), a series of biosensors has been developed to analyze mycotoxins in food in the last decade with the advantages of rapid analysis, simplicity, portability, reproducibility, stability, accuracy, and low cost. Nanomaterials have been incorporated into biosensors for the purpose of achieving better analytical performance in terms of limit of detection, linear range, analytical stability, low production cost, etc. Gold nanoparticles (AuNPs) are one of the most extensively studied and commonly used nanomaterials, which can be employed as an immobilization carrier, signal amplifier, mediator and mimic enzyme label. This paper aims to present an extensive overview of the recent progress in AuNPs in mycotoxin detection through ELISA and biosensors. The details of the detection methods and their application principles are described, and current challenges and future prospects are discussed as well.

Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

BACKGROUND: Wheat is one of three major food crops in China. Alternaria species can cause spoilage of wheat with consequent mycotoxin accumulation. Alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA) are the most common and frequently studied mycotoxins. There are limited regulations placed on Alternaria mycotoxin concentrations worldwide due to the lack of toxicity data available. More data on the levels of mycotoxin contamination are also needed. It is also important to reduce the risks of Alternaria mycotoxins. RESULTS: One hundred and thirty-two wheat samples were collected from Hebei Province, China, and analyzed for AOH, AME, and TeA. Tenuazonic acid was found to be the predominant Alternaria mycotoxin, especially in flour samples. Studying Alternaria species that cause black-point disease of wheat indicated that Alternaria alternata and Alternaria tenuissima were the dominant species. Most of the Alternaria strains studied produced more than one mycotoxin and TeA was produced at the highest concentration, which may have resulted in the high level of TeA contamination in the wheat samples. Furthermore, magnolol displayed obvious antifungal and antimycotoxigenic activity against Alternaria. This is the first report on the antimycotoxigenic activity of magnolol against Alternaria species. CONCLUSION: The Alternaria mycotoxin contamination levels in wheat and wheat products from Hebei Province, China, were correlated with the toxigenic capacity of the Alternaria strains colonizing the wheat. Considering its safety, magnolol could be developed as a natural fungicide in wheat, or as a natural alternative food preservative based on its strong antifungal and antimycotoxigenic activity against Alternaria strains. © 2020 Society of Chemical Industry.

Multiwalled Carbon Nanotube for One-Step Cleanup of 21 Mycotoxins in Corn and Wheat Prior to Ultraperformance Liquid Chromatography⁻Tandem Mass Spectrometry Analysis.

One-step solid-phase extraction (SPE) using a multiwalled carbon nanotube (MWCNT) for simultaneous analysis of 21 mycotoxins, including nine trichothecenes, zearalenone (ZEN) and its derivatives, four aflatoxins, and two ochratoxins, in corn and wheat was developed. Several key parameters affecting the performance of the one-step SPE procedure-types of MWCNT, combinations with five sorbents (octadecylsilyl (C18), hydrophilic⁻lipophilic balance (HLB), mixed-mode cationic exchange (MCX), silica gel, and amino-propyl (NH2)), and filling amounts of the MWCNTs-were thoroughly investigated. The combination of 20 mg carboxylic MWCNT and 200 mg C18 was proven to be the most effective, allowing the quantification of all analyzed mycotoxins in corn and wheat. Under the optimized cleanup procedure prior to ultraperformance liquid chromatography⁻tandem mass spectrometry (UPLC⁻MS/MS) analysis, the method was validated by analyzing samples spiked at the limit of quantification (LOQ), two-times LOQ, and 10-times LOQ. Satisfactory linearity (r² ≥ 0.9910), high sensitivity (LOQ in different ranges of 0.5⁻25 µg L-1), good recovery (75.6⁻110.3%), and acceptable precision (relative standard deviation (RSD), 0.3⁻10.7%) were obtained. The applicability of the method was further confirmed using raw samples of corn and wheat. In conclusion, the established method was rapid, simple and reliable for simultaneous analysis of 21 mycotoxins in corn and wheat.

Survey of Alternaria Toxins and Other Mycotoxins in Dried Fruits in China.

Occurrence of toxigenic molds and mycotoxins on dried fruits is a worldwide problem, but limited information is available in China. A total of 220 dried fruits (raisins, dried apricots, dates and wolfberries) purchased from China were analyzed for 17 mycotoxins (i.e., Alternaria toxins, ochratoxin A (OTA), patulin (PAT) and trichothecenes) by UPLC-MS/MS, combined with a single-step cleanup. The result showed that at least one mycotoxin was detected in 142 samples (64.6%). The lowest incidence of contaminated samples was observed in dried apricots (48.2%), and the highest incidence in dried wolfberries (83.3%). The Alternaria toxins seemed to be the major problem in dried fruits, rather than OTA or PAT. Tenuazonic acid (TeA) was the predominant mycotoxin, in both frequency and concentration, ranging from 6.9 to 5665.3 µg kg-1, followed by tentoxin (TEN; 20.5%), and mycophenolic acid (MPA; 19.5%). Moreover, raisins are more likely to be contaminated with OTA than the other dried fruits. Penicillic acid (PA) was detected only in dried dates, and PAT was detected only in one apricot sample. In addition, our results also showed that the simultaneous presence of 2-4 mycotoxins was observed in 31.4% of dried fruits. TeA and TEN were the most frequent combination, detected in 29 (13.2%) samples, followed by TeA and MPA with a prevalence of 11.4%. Therefore, the results of this survey suggest the need for wider monitoring on the contamination of these mycotoxins, especially Alternaria toxins in agro-products, and indicate the importance of setting a maximum limit for Alternaria toxins in China.

A single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry.

A simple and rapid extraction procedure for the simultaneous determination of eight mycotoxins (Alternaria toxins, ochratoxin A, patulin, citrinin) in a variety of fruit matrices has been developed using ultra high performance liquid chromatography coupled to tandem mass spectrometry. The procedure involves a one-step cleanup using homemade solid phase extraction (SPE) cartridges. By comparative evaluation among six various adsorbents (C18, PSA, HLB, MCX, Silica, NH2), the combination of MCX and NH2 was found to provide the most effective cleanup, removing the greatest number of matrix interferences and also allowing the quantification of all analyzed mycotoxins in fruits. The optimized extraction conditions including acidified aqueous acetonitrile and an additional salt-out step using NaCl were employed before SPE cleanup. Method validation was performed by analyzing samples spiked at three levels (LOQ, 2 LOQ and 10 LOQ). Four fruits including apple, sweet cherry, tomato and orange fruits were selected, and accuracy (recovery%), precision (RSD%), limits of quantification (LOQ), linearity and matrix effect were evaluated during validation. Matrix-matched linearity with correlation coefficients ≥ 0.9921 was established in the range of 5-200 ng mL(-1) for patulin and 1-200 ng mL(-1) for other mycotoxins, respectively. Recoveries between 74.2% and 102.4% and relative standard deviations lower than 4.7% were obtained for all tested fruits. The matrix effect observed was low (≤ ± 17%) in all three fruit matrixes with the exception of orange, for which strong ion suppression was observed for alternariol (25.3%), ochratoxin A (31.6%) and citrinin (40.3%). Therefore, matrix-matched calibration was used for a correct quantification in order to compensate for matrix effect. The limits of quantification (LOQ), ranging from 1 to 5 µg kg(-1) depending on mycotoxins type, were always lower than maximum permitted levels for every regulated mycotoxin by the current European legislation.